Staff Research Associate I - Ophthalmology (Limited)

Under the direct supervision of the Principal Investigator
and a Post-doctoral Scholar, the Staff Research Associate I will use tissue
culture techniques, molecular biology, cell biology, and biochemistry in
projects to develop and maintain induced pluripotent stem cell-derived eye
tissues for use in the study of inherited diseases of the eye.

This is a limited position that may convert to
career.

Salary Range: $30.12 - $35.92 Hourly

All Required:

• Knowledge
  and skill in RPE cell culture
• Knowledge
  of RPE cell physiology and protein expression patterns
• Skill
  in immunohistochemical and immunofluorescent staining
• Skill
  in confocal microscopy
• Skill
  in protein quantitation
• Skill
  in gel electrophoresis
• Skill
  in Western blot analyses
• Skill
  in DNA/RNA isolations
• Knowledge
  and skill in DNA clonin
• Skill
  in constructing expression vectors
• Skill
  in handling, applying and assessing CRISPR/Cas-9 vector systems and
  applications in RPE diseases
• Skill
  in Super-resolution imaging in immunofluorescence of organelles
• Skill
  in good verbal communication
• Skill
  in searching for literature relevant to research projects
• Ability
  to follow protocol accurately
• Ability
  to use PC/MAC, Microsoft Word, Excel, and PowerPoint
• Ability
  to work as part of a team, collaborating with other members of the laboratory
  if necessary